Structure analysis of the telomere resolvase from the Lyme disease spirochete Borrelia garinii reveals functional divergence of its C-terminal domain

Author:

Semper, C, Watanabe, N, Karimullina, E, Patel, D, Di Leo, R, Castellanos, M, Patel, D, and Chaconas, G

Array:

Publication type:

Journal Article

Publication date:

2024-07

Journal/Series title:

Nucleic Acids Res

URL:

https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae580/7709540?log...

Abstract:

Our data provides the first high-resolution structures of ResT from Borrelia spirochetes and revealed active site positioning in the catalytic domain. We also demonstrate that the C-terminal domain of ResT is required for both transesterification steps of telomere resolution, and is a requirement for DNA binding, distinguishing ResT from other telomere resolvases from phage and bacteria. These results advance our understanding of the molecular function of this essential enzyme involved in genome maintenance in Borrelia pathogens.