Minimal mutational requirements for conversion of a telomere resolvase into a Cre-like site-specific recombinase

Author:

Huang, S, and Kobryn, K

Publication type:

Journal Article

Publication date:

2026-05

Journal/Series title:

PloS One

Volume:

Issue:

URL:

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0350834

Abstract:

A complex combination of autoinhibition domain deletion and point mutation of TelA had even been shown to completely switch the activity of TelA from a telomere resolvase to a recombinase. We report here that mutation of a pair of aspartic acid residues in TelA is sufficient to accomplish this switch in activity.