Construction and characterization of a Borrelia burgdorferi strain with conditional expression of the essential telomere resolvase, ResT.

Author:

Bandy, NJ, Salman-Dilgimen, A, and Chaconas, G

Publication type:

Journal Article

Publication date:

2014-07

Journal/Series title:

J Bacteriol.

Volume:

196

Issue:

URL:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4054159/

Abstract:

Borrelia species are unique in the bacterial world in possessing segmented genomes which sometimes contain over 20 genetic elements. Most elements are linear and contain covalently closed hairpin ends requiring a specialized process, telomere resolution, for their generation. Treatment of DNA from the ResT-depleted strain with ResT in vitro revealed the presence of replicated telomeres expected in replication intermediates.