Analysis of an Ordered, Comprehensive STM Mutant Library in Infectious Borrelia burgdorferi: Insights into the Genes Required for Mouse Infectivity.

Author:

Lin, T, Gao, L, Zhang, C, Odeh, E, Jacobs, MB, Coutte, L, Chaconas, G, Phillipp, MT, and Norris, SJ

Publication type:

Journal Article

Publication date:

2012-10

Journal/Series title:

PLoS ONE

Volume:

Issue:

URL:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0047532

Abstract:

Using the infectious, moderately transformable B. burgdorferi derivative 5A18NP1 and signature-tagged versions of the Himar1 transposon vector pGKT, we have constructed a defined transposon library for the efficient genome-wide investigation of genes required for wild-type pathogenesis, in vitro growth, physiology, morphology, and plasmid replication.