RNA-Seq Analysis of Gene Expression, Viral Pathogen, and B-Cell/T-Cell Receptor Signatures in Complex Chronic Disease.

Author:

Bouquet, J, Gardy, JL, Brown S, Pfeil, J, Miller, RR, Morshed, M, Avina-Zubieta, A, Shojania, K, McCabe, M, Parker, SM, Uyaguari, M, Federman, S, Tang, P, Steiner, TS, and Otterstater, M

Publication type:

Journal Article

Publication date:

2017-02

Journal/Series title:

Clin Infect Dis.

Volume:

Issue:

URL:

https://www.ncbi.nlm.nih.gov/pubmed/28172519

Abstract:

Here, we performed blinded RNA-seq analysis of whole blood collected from 25 adults diagnosed with CFS and 13 ADCLS patients, comparing these cases to 25 matched controls and 11 patients with well-controlled systemic lupus erythematosus (SLE). Samples were collected at patient enrollment and not during acute symptom flares. RNA-seq data were used to study host gene expression, B-cell/T-cell receptor profiles (BCR/TCR), and potential viral infections.