Differential detection of tick-borne pathogens in human platelets and whole blood using microfluidic PCR

Author:

Banovic, P, Piloto-Sardinas, E, Mihatovic, D, Foucault-Simonin, A, Simin, V, Bogdan, I, Obregon, D, Mateos-Hernandez, L, Moutallier, S, and Cabezas-Cruz, A

Publication type:

Journal Article

Publication date:

2022-11

Journal/Series title:

Acta Trop

URL:

https://www.sciencedirect.com/science/article/abs/pii/S0001706X22004478?via%3Dih...

Abstract:

Overall, nine different pathogens with variable prevalence were identified using species-specific primers, and the most common was Rickettsia helvetica. In three patients, there were no coincidences between the TBPs detected in whole blood and tick samples. Only in one patient was detected A. phagocytophilum in both, whole blood and tick samples. These results suggest the unequal detection of TBPs in whole blood, platelet fraction and ticks collected, from the same individual. The results justify the use of both whole blood and platelet fraction for molecular diagnosis of TBPs in patients.