Biosensor for Lyme Disease: Real-time, Specific, and Label-Free Complementary Metal-Oxide-Silicon Transistor Sensing of Borrelia burgdorferi s.l. Outer Surface Protein A in Unprocessed Blood
The study demonstrates OspA sensing with a limit of detection of 1 fg/mL, a dynamic range of 8 orders of magnitude, and with excellent sensitivity and linearity. The MNC-FET biosensor is fabricated in a complementary metal-oxide-silicon (CMOS) process ensuring superb electronic grade in terms of noise levels and amplification, robustness, stability, and ultimate miniaturization suitable for future multiplexed sensing. The unique MNC-FET biosensor design addressing challenges associated with molecular sensing, coupled with CMOS technology potential for high volume production of high-end chips, provides a viable technology for a low-cost Lyme disease diagnostic kit with excellent quantitative performance in ultra-small unprocessed blood drops.