Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada

TitleUsing Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada
Publication TypeJournal Article
Year of Publication2023
JournalEmerg Infect Dis
Volume29
Issue1
Start Page175
Date Published01/2023
AuthorsBoodman C, Loomer C, Dibernardo A, Hatchette TF, LeBlanc J, Waitt B, Lindsay LR
KeywordsAnaplasma phagocytophilum, anaplasmosis, bacteria, Canada, diagnostics, emerging infections, PCR, ticks, vector-borne infections, zoonoses
Abstract

Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%-95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable.
URLhttps://wwwnc.cdc.gov/eid/article/29/1/22-0988_article