Possible effect of mutations on serological detection of Borrelia burgdorferi sensu stricto ospC major groups: An in-silico study

TitlePossible effect of mutations on serological detection of Borrelia burgdorferi sensu stricto ospC major groups: An in-silico study
Publication TypeJournal Article
Year of Publication2023
JournalPLoS One
Volume18
Issue10
Start Pagee0292741
Date Published10/2023
AuthorsMechai S, Coatsworth H, Ogden NH
Keywordsantibodies, diagnostic medicine, nucleotide sequencing, phylogenetic analysis, phylogenetics, polynomials, serodiagnosis, single nucleotide polymorphisms
Abstract

Regression analysis was conducted using genetic distance for the full ospC sequence, and the subsections individually. There was a significant association between the reactivity difference and genetic distance of ospC MGs from ospC A: increased genetic distance reduced reactivity to OspC A. No single ospC subsection sequence fully explained the relationship between genetic distance and reactivity difference. An analysis of single nucleotide polymorphisms supported a biological explanation via specific amino acid modifications likely to change protein binding affinity. This adds support to the hypothesis that genetic diversity of B. burgdorferi s.s. (here specifically OspC) may impact serological diagnostic test performance. Further prospective studies are necessary to explore the clinical implications of these findings.

URLhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0292741