Differential detection of tick-borne pathogens in human platelets and whole blood using microfluidic PCR

TitleDifferential detection of tick-borne pathogens in human platelets and whole blood using microfluidic PCR
Publication TypeJournal Article
Year of Publication2022
JournalActa Trop
Start Page106756
Date Published11/2022
AuthorsBanovic P, Piloto-Sardinas E, D, M, Foucault-Simonin A, Simin V, Bogdan I, Obregon D, Mateos-Hernandez L, Moutailler S, Cabezas-Cruz A
Keywordsdiagnosis, platelets, tick-borne pathogens, ticks, whole blood
Abstract

Overall, nine different pathogens with variable prevalence were identified using species-specific primers, and the most common was Rickettsia helvetica. In three patients, there were no coincidences between the TBPs detected in whole blood and tick samples. Only in one patient was detected A. phagocytophilum in both, whole blood and tick samples. These results suggest the unequal detection of TBPs in whole blood, platelet fraction and ticks collected, from the same individual. The results justify the use of both whole blood and platelet fraction for molecular diagnosis of TBPs in patients.

URLhttps://www.sciencedirect.com/science/article/abs/pii/S0001706X22004478?via%3Dihub