The application of next-generation sequence-based DNA barcoding for bloodmeal detection in host-seeking wild-caught Ixodes scapularis nymphs

TitleThe application of next-generation sequence-based DNA barcoding for bloodmeal detection in host-seeking wild-caught Ixodes scapularis nymphs
Publication TypeJournal Article
Year of Publication2021
AuthorsLumsden G, Zakharov EV, Dolynskyj S, Weese JS, Lindsay LR, Jardine CM
JournalBMC Res Notes
Volume14
Issue1
Start Page67
Pagination67
Date Published02/2021
Keywordsblacklegged tick, bloodmeal analysis, DNA barcoding, host identification, Next-generation sequencing
Abstract

Bloodmeal identification was unsuccessful in most samples (99% of 216 specimens) demonstrating a very low detection rate of this assay. Sequences that surpassed quality thresholds were obtained for 41.7% of nymphs (n = 90) and of those, confident species identification was obtained for 15.6% of nymphs (n = 14). Wild host identifications were only obtained from 2 specimens, where DNA from the eastern grey squirrel (Sciurus carolinensis) was identified. Human and bovine DNA was identified in remaining nymphs and considered to be contaminants. Further optimization of the technique is required to improve detection of remnant bloodmeals in host-seeking nymphs.

URLhttps://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-021-05481-3