|Title||The application of next-generation sequence-based DNA barcoding for bloodmeal detection in host-seeking wild-caught Ixodes scapularis nymphs|
|Publication Type||Journal Article|
|Year of Publication||2021|
|Authors||Lumsden G, Zakharov EV, Dolynskyj S, Weese JS, Lindsay LR, Jardine CM|
|Journal||BMC Res Notes|
|Keywords||blacklegged tick, bloodmeal analysis, DNA barcoding, host identification, Next-generation sequencing|
Bloodmeal identification was unsuccessful in most samples (99% of 216 specimens) demonstrating a very low detection rate of this assay. Sequences that surpassed quality thresholds were obtained for 41.7% of nymphs (n = 90) and of those, confident species identification was obtained for 15.6% of nymphs (n = 14). Wild host identifications were only obtained from 2 specimens, where DNA from the eastern grey squirrel (Sciurus carolinensis) was identified. Human and bovine DNA was identified in remaining nymphs and considered to be contaminants. Further optimization of the technique is required to improve detection of remnant bloodmeals in host-seeking nymphs.