|Title||Analysis of recombinational switching at the antigenic variation locus of the Lyme spirochete using a novel PacBio sequencing pipeline.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Verhey TB, Castellanos M, Chaconas G|
|Keywords||antigenic, borrelia, lyme, proteins, spirochete, surface, switching, variation|
Here we use PacBio sequencing technology coupled with the first fully-automated software pipeline (VAST) to accurately process NGS data by minimizing error frequency, eliminating heteroduplex errors and accurately aligning switch variants. We extend earlier studies by showing use of almost all of the vlsE SNP repertoire. In different tissues of the same mouse, 99.6% of the variants were unique, suggesting that dissemination of Borrelia burgdorferi is predominantly unidirectional with little tissue-to-tissue hematogenous dissemination.