Performance of a Modified Two-Tiered Testing (MTTT) EIA Algorithm for Serologic Diagnosis of Lyme Disease in Nova Scotia

TitlePerformance of a Modified Two-Tiered Testing (MTTT) EIA Algorithm for Serologic Diagnosis of Lyme Disease in Nova Scotia
Publication TypeJournal Article
Year of Publication2020
AuthorsDavis IRC, McNeil SA, Allen W, MacKinnon-Cameron D, Lindsay LR, Bernat K, Dibernardo A, LeBlanc J, Hatchette TF
JournalJ Clin Microbiol
VolumeJCM.01841-19
Date Published04/2020
Abstract

Compared to the standard two-tier testing algorithm (STTT) for Lyme disease serology using an enzyme immunoassay (EIA) followed by Western blot, data from the United States (US) suggests that a modified two-tiered testing (MTTT) algorithm employing two EIAs has improved sensitivity to detect early localized Borrelia burgdorferi infections, without compromising specificity. From 2011-2014, in the Canadian province of Nova Scotia where Lyme disease is hyperendemic, sera submitted for Lyme disease testing was subjected to a whole cell EIA, followed by C6 EIA and subsequently IgM and/or IgG immunoblots on sera with EIA positive or equivocal results. Here we evaluate the effectiveness of the MTTT algorithm compared to the STTT approach in a Nova Scotian population.

URLhttps://jcm.asm.org/content/early/2020/04/17/JCM.01841-19/article-info